Glycan Analysis
GlycoUniverse's expertise in post-automation carbohydrate synthesis and purification techniques enable us to offer individualized approaches to study our client's compounds of interest and/or their accompanying isomers/impurities.
Released glycan profiling
Glycosylation of is one of the most common post-translational modifications and, unlike transcription, the final glycan profile is heavily dependent on changes in biochemical environments and competition between glycosyltransferases for the same substrate. Monitoring the glycan profile is therefore required to ensure consistency and reduce risk of unintended effects.
(U)HPLC HILIC & WAX profiling
The hydrophilic interaction chromatography (HILIC) of native and/or fluorophore labelled N- and O-glycans is one of the most used methods for glycan analysis. Direct comparison of sample to GlycoUniverse's glycan standards reveals preliminary identification of glycan epitopes. For negative charged molecules, such as sialic acid-containing glycans, the weak anion exchange chromatography (WAX) is another important method to analyze charge profile.
In-depth structural characterization
Nuclear magnetic resonance (NMR) spectroscopy is a non-destructive and non-invasive technique, that can be used to determine molecular structure and molecular dynamics. Natural or custom-made synthetic glycans containing NMR-active nuclei such as 1H, 13C, 15N, 31P, 23Na, and 19F provide unprecedented insights into glycan-protein, glycan-lipid, glycan-nucleotide interactions.